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Confocal Scanning Optical Microscopy and Related Imaging Systems

Corle, Timothy R.; Kino, Gordon S.
ISBN-10: 0124087507
ISBN-13: 9780124087507

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This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. These microscopes have been designed to overcome the problems associated with submicrometer imaging of complex three-dimensional structures. The book concentrates mainly on two of these instruments: the confocal scanning optical microscope (CSOM), and the optical interference microscope (OIM).
In these instruments a defocused image disappears rather than blurs as it does in a standard microscope. As a result, researchers can visualize submicrometer structures, determine their surface profiles, and observe a selected cross section of transparent materials withut cutting the sample into thin slices. A comprehensive discussion of the theory and design of the near-field scanning optical microscope (NSOM) is also given. The text also discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope and also considers the applications of these instruments to phase imaging, biological imaging, and semiconductor inspection and metrology. A comprehensive theoretical discussion of the depth and transverse resolution is included, with emphasis placed on the practical results of the theoretical calculations and their uses in understanding the operation of these microscopes.This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. the text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes.This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Key Features * Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers * Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology * Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations * Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications * Discusses the theory and design of near-field optical microscopes * Explains phase imaging in the scanning optical and interference microscopes
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Preface
Introduction
Confocal and Interferometric Microscopy
The Standard Optical Microscope
Principle of Operation
The Point Spread Function
Coherent and Incoherent Illumination
The Coherent Transfer Function, Line Spread Function, and Spatial Frequencies
The Optical Transfer Function
The Rayleigh and Sparrow Two-Point Definitions
Brightness of the Image
Imaging Techniques with the Standard Optical Microscope
The Confocal Microscope
Principle of Operation
Scanning
Depth Response
The Point Spread Function and Two-Point Resolution
History of the CSOM
Optical Interference Microscopes
Principle of Operation
Signal Processing Techniques
Depth and Transverse Resolution
Comparison of Scanning Optical Microscopes with Other Types of Scanning Microscopes
References
Instruments
Introduction
The Confocal Scanning Laser Microscope
The Illumination Source
The Objective Lens
The Scanning Stage
The Intermediate Optics
The Pinhole
The Detector and Electronics
Beam Scanning Techniques
Commercial Examples
Fiber-Optic Scanning Microscopes
Nipkow Disk Scanning Microscopes
One-Sided and Two-Sided Designs
The Nipkow Disk
Illumination of the Disk
The Tilted Disk and Optical Isolator
The Field Lens, Tube Lens, and Objective Lens
The Imaging Path
Commercial Examples
Slit Microscopes
Ophthalmologic Slit Microscopes
Bilateral Scanning Slit Microscopes
Hybrid Slit Microscopes
Confocal Transmission Microscopes
Alternative Imaging Configurations
Interference Microscopes
Interference CSOMs
The Michelson Interference Microscope
The Linnik Interference Microscope
The Mirau Interference Microscope
The Tolanski Interference Microscope
Near-Field Microscopy
The Near-Field Scanning Optical Microscope
Applications of the NSOM
The Solid Immersion Microscope
Conclusion
References
Depth and Transverse Resolution
Introduction
Depth Response of the Confocal Microscope with Infinitesimal Pinholes and Slits
Scalar Theory for a Plane Reflector
Scalar Theory for Depth Response of a Point Reflector
Scalar Theory for Fluorescent Reflectors
Scalar Theory for Confocal Slit Microscopes
The Effect of Sample and Lens Aberrations on the Depth Response
Depth Response of the Confocal Microscope with Finite-Sized Pinholes
Approximate Theory for Optimum Pinhole Size
Approximate Theory for the Range Resolution vs. Pinhole Size
Exact Theory for the Range Resolution vs. Pinhole Size
Transverse Response of the Confocal Microscope
Transverse Response for Infinitesimal Pinholes
Two-Point Resolution
Edge and Line Response
The Effect of Finite Pinhole Size on the Transverse Resolution
Depth and Transverse Resolution of the Interferometric Microscope
Scalar Theory for the Depth Response with a Plane Reflector
Transverse Resolution
The Effect of the Thin-Film Beamsplitter and Mirror Support of the MCM on Signal Levels, Range, and Transverse Resolution
The Near-Field Scanning Optical Microscope (NSOM)
Attenuation in a Tapered Rod or Fiber
The Fields outside the Pinhole
The Solid Immersion Microscope (SIM)
The Transverse and Longitudinal Magnifications of the SIL
The Depth Response of the SIM
The Transverse Response of the SIM
Conclusion
References
Phase Imaging
Introduction
Phase-Contrast Imaging in Conventional Microscopes
Phase-Contrast Imaging in the CSOM
Phase Imaging with an Interferometer
Electro-optic Phase Imaging
The ac Zernike Technique
Acousto-optic Phase Imaging
Differential Interference Contrast Imaging
The Basic Theory of Nomarski Imaging
Imaging Modes of a DIC Microscope
Polarization-Shifted DIC Imaging
Split Detector DIC Imaging
Differential Probe Beam DIC Imaging
Differential Imaging with an AO Modulator
Differential Imaging with an Optical Fiber CSOM
Phase Imaging with an Interference Microscope
The Integrating Bucket Technique
The Fourier Transform Technique
Conclusion
References
Applications
Introduction
Semiconductor Metrology
Microlithography Measurements
Precision, Linearity, and Accuracy in Semiconductor Metrology
Critical Dimension Measurements
Experimental Results
Polarization-Enhanced Imaging of Dense Arrays
Calibration
Overlay Misregistration Measurements
Film Thickness Measurements
CARIS and VAMFO
Film Thickness Measurements with the Mirau Interference Microscope
Biological Imaging
Brightfield and Phase Imaging
Fluorescence Imaging
Two-Wavelength and Two-Photon Fluorescence Imaging
Conclusion
References
Vector Field Theory for Depth and Transverse Resolution of a CSOM
The Depth Response
Transverse Response
References
Index



Edition: 1996
Publisher: Elsevier Science & Technology Books
Binding: Trade Cloth
Pages: 335
Size: 6.25" wide x 9.50" long x 0.75" tall
Weight: 1.43 lbs.
Language: English

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