Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and tricks of the trade to experienced researchers and more junior members in the lab. * Provides busy researchers a quick reference for time-tested methods and protocols that really work. * Includes quick tips and tricks for each method. * Provides pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error.The best techniques and practices for researchers using zebrafish as a research model as compiled by the editors of the well-known Methods in Cell Biology series.Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
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| Overview of the Zebrafish System | |
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| Detrich III Cell Cycles and Development in the Embryonic Zebrafish | |
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| Primary Fibroblast Cell Culture | |
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| Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) | |
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| Analysis of Protein and Gene Expression Trevor Jowett Analysis of Zebrafish Development Using Explant Culture Assays Hazel Sive Confocal Microscopic Analysis of Morphogenetic Movements | |
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| Cytoskeletal Dynamics of the Zebrafish Embryo Lilianna Solinica-Krezel Haploid Screens and Gamma-Ray Mutagenesis Charline Walker Positional Cloning of Mutated Zebrafish Genes Will Talbot The Zebrafish Genome | |
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| Analyzing Axon Guidance in the Zebrafish Retinotectal System | |
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| Analysis of the Cell Cycle in Zebrafish Embryos | |
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| Cellular Dissection of Zebrafish Hematopoiesis | |
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| Culture of Embryonic Stem Cell Lines from Zebrafish | |
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| Neurogenesis Laure Bally-Cuif Time-Lapse Microscopy of Brain Development | |
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| Development of the Peripheral Sympathetic Nervous System in Zebrafish | |
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| Approaches to Study Neurogenesis in the Zebrafish | |
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| Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms | |
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| Development of Cartilage and Bone | |
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| Morphogenesis of the Jaw: Development Beyond the Embryo | |
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| Cardiac Development Didier Y. R. Stainier Zebrafish Kidney Development | |
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| Retroviral-Mediated Insertional Mutogenesis in Zebrafish | |
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| Genetic Screens for Maternal-Effect Mutations | |
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| Behavior Screening Assays in Zebrafish | |
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| A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish | |
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| Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element | |
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| Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in Zebrafish | |
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| The Zon Laboratory Guide to Positional Cloning in Zebrafish | |
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| Sleeping Beauty Transposon for Efficient Gene Delivery | |
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| Transgene Manipulation in Zebrafish by Using Recombinases | |
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| Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt Cloning Zebrafish by Nuclear Transfer Shuo Lin Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale | |
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| In Situ Hybridization Screening Christine Thisse Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow Common Diseases of Laboratory Zebrafish | |
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| Zebrafish Sperm Cryopreservation | |
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Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
