Presenting the fundamental principles behind the various FRET microsopy & spectroscopy techniques available, this book covers the preparation of specimens & the selection & use of the best experimental approaches to specific biological questions.The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology.
With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind thevarious FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuableinformation about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRETexperiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.This is a gold mine of information for those interested in using FRET microscopy to study protein-protein interactions. The book is clearly written, carefully edited, and engaging."This is a gold mine of information for those interested in using FRET microscopy to study protein-protein interactions. A major strength of this book is that it is focused and yet comprehensive...The book is clearly written, carefully edited, and engaging...It will be a valued resource and practical guide for years to come...5 Stars! (Highest Rating)"--Doody'sFRET microscopy and spectroscopy techniques provide the tools for measuring protein interactions within living cells at the nanomolecular level. This unique book clearly presents the fundamental principles behind the various FRET microscopy and spectroscopy techniques, and is an indispensable source of practical information about the preparation of specimens and the selection and use of the best experimental approaches to address specific biological questions.
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