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Imaging Mass Spectrometry Protocols for Mass Microscopy

Setou, Mitsutoshi
ISBN-10: 4431094245
ISBN-13: 9784431094241

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Description

Table of Contents

Author Bios

Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. It provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, data analysis, and more.

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Innovation of Imaging Mass Spectrometry



IMS as an Historical Innovation




Planning the Sample Preparation



Guide to Planning the Sample Preparation Step




Sample Preparation



Animal Care and Tissue Sample Extraction for IMS




Preparing Biological Tissue Sections for Imaging Mass Spectrometry




Matrix Choice




Methods of Matrix Application




Matrix Application with ImagePrep




Instrumental Operation for IMS



Method of Operating AXIMA-QIT as Imaging Instrument




Analysis of Measurement Data



Ion Image Reconstruction Using BioMap Software




Statistical Procedure for IMS Data Analysis




Statistical Analysis of IMS Dataset with ClinproTool Software




Applications of MALDI-IMS



Imaging of Cultured Cells by Mass Spectrometry




TLC-Blot-MALDI-IMS




IMS Applications Provided by Manufacturers



Applied Biosystems




Bruker Daltonics




Imaging and Molecular Identification of Biomolecules on Tissue Sections with AXIMA-QIT: Shimadzu Corporation




MALDI Imaging with Ion-Mobility MS: Waters Corporation




Comparison of SIMS and MALDI for Mass Spectrometric Imaging



Comparison of SIMS and MALDI for Mass Spectrometric Imaging



Index

Edition:	2010
Publisher:	Springer
Binding:	Trade Paper
Pages:	272
Size:	6.25" wide x 9.25" long x 0.50" tall
Weight:	1.03 lbs.
Language:	English

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